Keywords
Abstract
One of the most important technology stages, on which the efficiency of propagation process directly depends, is the adaptation of plants in vitro to ex vitro conditions. The main task of adaptation is to mitigate stress loading and ensure smooth transition to cultivation in new conditions. The aim of the study is to determine the factors, ensuring effective adaptation of grape plants in vitro to ex vitro conditions using the example of the rootstock variety ‘Kober 5 BB’ (‘Berlandieri x Riparia Kober 5BB’). Using of white daylight lamp and phytolamp for additional illumination is a positive factor for accelerating adaptation processes
to ex vitro conditions, contributed to normalization of photosynthesis, stimulation of morphogenesis and high survival ability. Using of a substrate based on 100% high-moor peat with acidity of pH 5.6–6.5 made it possible to achieve 100% survival rate at the stage of adaptation without additional nutrition. At the stage of completing growing of adapted plants, it is also advisable to use peat as a substrate due to its antiseptic and bactericidal properties, but with obligatory extra-nutrition. In general, adaptation in late summer followed by planting to complete growing in early autumn has a positive development.