Keywords
microsatellite loci
nSSr
cpSSr
allelic polymorphism
molecular-genetic passports
Abstract
identification and control of genetic variation of different varieties of
agricultural crops enter as prerequisites for their efficient conservation
and use. Genetic variation is investigated by means of a wide set of
methods, including those relying on molecular-genetic analysis. Every
year, dozens of new breedings of grapevine come into being, and their
passportization is necessary. Molecular markets can promote selection
of parent pairs for crossing, improve efficiency of breeding and accelerate the process as enable both identification of the initial material and
analysis of crossing results to be done in a sufficiently short period of time.
the highest information value is associated with microsatellite markers
consisting of simple sequence repeats (SSr). analysis of polymorphism
of SSr loci allows to investigate genetic variation of agricultural crops at
the level of genome. the goals of the study were to conduct genotyping
of a number of grape breedings developed by the institute Magarach, to
assess their allelic diversity and to achieve dna passportization based
on SSr analysis. Polymerase chain reaction (Pcr) and fragment analysis
of Pcr products with the use of a genetic analizer aBi 3130 were the
main methods the study relied upon. as a result of the aforesaid analysis,
eight new breedings of the institute were genotyped for nine nuclear
microsatellite loci (nSSr). the sizes of alleles were assessed using Gene
Mapper v. 4.0 software. Popgene (v. 1.32) software was used to calculate
polymorphism of microsatellite loci and genetic diversity. a comparative
nSSr-analysis of dna-profiles of the study varieties indicated that all of
them had unique profiles. a total of 69 alleles were identified, with 7.67
alleles per locus on an average. Based on the sizes of the alleles, individual
molecular-genetic passports of the varieties were made, in accordance
with international standards.