Изучение полиморфизма экзона гена VvAGL11 сорта винограда Кишмиш черный на основе результатов секвенирования нового поколения (NGS) биоинформатическими методами

Based on the results of NGS of the genome of ancient variety ‘Kishmish Chernyi’ using bioinformatic methods, the sequence of VvAGL11 (chr18) gene locus, as a candidate gene for seedlessness trait in grapes, was obtained. In this work, the polymorphism of exon 7 of the VvAGL11 gene, where SNP 26 889 437 was previously described, is studied, which is supposed to lead to seedlessness as a result of a missense mutation - the replacement of amino acids arginine with leucine. In the sequence of the studied seedless variety, compared with the reference sequence of the seeded one ‘Pinot Noir’, in the 200 bp locus under consideration, which includes exon 7 and partially introns 6 and 7 of the VvAGL11 gene, eight SNPs were found without insertions and deletions, five SNPs directly in exon 7. Seven of eight found SNPs were previously described for the seedless variety ‘Sultanina’ and the cultivars derived from it. Causing the missense mutation target SNP 26 889 437 (A/C) was confirmed. Seeded variety ‘Pinot Noir’ in this position is homozygous (C/C). In the SNPs found in exon 7, seedless varieties ‘Kishmish Chernyi’ and ‘Sultanina’ are heterozygous, by contrast with the seeded one ‘Pino Noir’, which is homozygous. The sequence of ‘Kishmish Chernyi’ variety in the considered locus of the VvAGL11 gene is identical to the sequence of ‘Sultanina’
variety, except for the position 26 889 350 (intron 7), in which the studied variety carries heterozygote (T/C), and ‘Sultanina’ variety - homozygote (T/T). In this study, based on the genotype of seedless variety ‘Kishmish Chernyi’, the conclusion of Royo C. et al. (2018) on the SNP presence in the position chr18: 26 889 437 in all seedless varieties, which is a hypothetic cause of grape seedlessness, is confirmed.